LSFM2019, this year’s big light-sheet microscopy conference in Frankfurt, is organized by Francesco Pampaloni and Ernst H.K. Stelzer, of the LSFM4LIFE coordinator team at the Buchmann Institute for Molecular Life Sciences (Goethe University Frankfurt, Germany), so it doesn’t come as a suprise that LSFM4LIFE is powering this event. And of course, our project is sending one of their microscopy experts to present the most recent research results: Till Moreth will give a talk in the session on “Organoids and 3D Cultures” (5 December 2019 at 12:55pm).

His presentation on “In toto analysis of pancreatic organoids reveals high intracultural heterogeneity” will address the question of why individual organoids behave so differently within the same culture.

Mouse pancreas organoids recorded by Till Moreth using a Zeiss Lightsheet Z.1 with a W Plan-Apochromat 20x/1.0 objective lens (fluorophore: dtTomato H2B YFP)

Till Moreth, who started his bachelor studies at Goethe University in 2009 and who has already been working on his master thesis in Ernst Stelzer’s lab, has been a predoc in Francesco Pampaloni’s team since 2016 and has been responsible for LSFM4LIFE’s establishment of image-based analysis of pancreatic organoid growth in close collaboration with Lotta Hof, another PhD student in the LSFM4LIFE team.

His recent research was dedicated to the study of mechanical forces in growing pancreatic organoids. He analyzed the growth of mouse and human pancreatic organoids, and showed that the acto-myosin system was not the only reason for an organoid’s shape and stability. In fact, he discovered that the traction force linker PIEZO1 in an inactivated state and the activated CFTR ion channel were essential for the luminal character and the liquid influx into the lumen. These results were presented on a poster that he showed at EOS2019 in Milan and at the “Goodbye Flat Biology” conference in Berlin, where this was combined with a flash talk.